Journal: bioRxiv

Article Title: The metabolic reprogramming of T cells controls airway remodeling in severe asthma

doi: 10.64898/2026.03.19.712985

Figure Lengend Snippet: To model EA or MGA respectively, Balb/c or Il4ra -/- mice were given five intranasal (i.n.) challenges with 100ug house dust mite (HDM) extract every week (followed by two days resting) for three consecutive weeks. A final i.n. HDM challenge was given 24 hours prior to harvest. ( A ) Lung sections were stained and representative Masson trichrome (collagen), α-smooth muscle actin (αSMA), and hematoxylin and eosin (H&E) sections and accompanying quantifications are shown. ( B ) Airway resistance to increasing doses of methacholine exposure (6-8 mice per group). (C) Expression of mRNA for IL13 and IL17, assessed by quantitative PCR analyses of lung samples. mRNA expression was calculated relative to GAPDH for 5 mice per group. ( D ) Number of neutrophils or eosinophils in the left lung lobe of EA or MGA induced mice, as assessed by flow cytometry. ( E ) MGA-induced animals were treated with 50ug dexamethasone intratracheally one hour prior to each allergen challenge, beginning on day 7. Lung sections were stained and representative Masson trichrome and αSMA sections and accompanying quantifications are shown. ( F ) Number of neutrophils or eosinophils in the left lung lobe of MGA induced mice treated with or without dexamethasone, as assessed by flow cytometry. ( G ) MGA-induced animals were treated with 100ug anti-IL17A or matching isotype control i.p. beginning on day 7 and continuing every other day until harvest. Lung sections were stained, and representative H&E sections and accompanying quantifications are shown. Trichrome data show area of interest values covering full lung section of 6 individual mice per condition. αSMA data show mean values of all bronchi scored from 6 mice per condition. Data are representative of 3 independent experiments. *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.00005; HPF, high-power field; ns, nonsignificant.

Article Snippet: To block IL-17 signaling, 100ug anti-IL17A (17F3; BioXCell) or matching isotype control (MOPC-21; BioXCell) were given intraperitoneally (i.p.) to HDM induced Ilr4a -/- mice beginning on day 7 and continuing every other day for a total of 7 injections.

Techniques: Staining, Expressing, Real-time Polymerase Chain Reaction, Flow Cytometry, Control

Journal: Science Advances

Article Title: Type 2 lymphocytes restrict type 3 lymphocytes during liver fibrosis and colocalize in fibroblast niches

doi: 10.1126/sciadv.aea6805

Figure Lengend Snippet: ( A and B ) Thick section confocal imaging from livers of RORγtGFP; Il5tdtomato- Cre/+; Rosa26 RFP/+ mice at the steady state (A) or after 4-week CCl 4 treatment or 14 days post-BDL (B). Higher magnification shows RORγtGFP + cells near IL-5 + lymphocytes (yellow arrows). PV (zone 1). Images represent two experiments; n = 7 or 8 mice per group. ( C ) Quantification of RORγt + cells <60 μm from Col1; n ≥ 3 mice per group. ( D ) Confocal imaging of control and 4-week CCl 4 –treated IL-17A lineage tracker mice (Il17atdtomato- Cre/+; Rosa26 RFP/+ ). n ≥ 4 mice per group. ( E ) IL-17 + lymphocytes per tissue volume. n = 4 or 5 mice per group. ( F ) Flow gating scheme for liver RORγtGFP + cells from reporter mice. ( G ) Quantification of RORγtGFP + subsets among CD45 + leukocytes in vehicle- or CCl 4 -treated mice. n ≥ 4 mice per group. ( H ) Percent RORytGFP + liver cells from reporter mice ± 4-week CCl 4 treatment; pooled from two experiments; n ≥ 9 mice per group. ( I ) PDGFRα + Sca1 + lung AFs cultured for 6 to 7 days with lung ILC2s and γδ T cells; TGF-β added to induce MF differentiation. n = 3 experiments. ( J ) Tbx21-ZsGreen “Tbet” reporter schematic, relevant to (J) to (M), and representative liver image after 4-week CCl 4 treatment or vehicle. Two mice per group, two sections each. ( K ) Perivascular IL-5 + , IL-17A + , and Tbet + lymphocytes: total; periportal <60 μm from α-SMA + , Col1a low or Col1a − , GS − ; and pericentral <60 μm from α-SMA low , Col1a + , GS + . n = 4 to 6 mice per group. ( L ) Percent Tbet + cells <60 μm from Col1. n = 2 to 4 mice per group. ( M ) Flow gating scheme for liver ZsGreen expression in Tbet-ZsGreen mice after 4-week CCl 4 treatment. All scale bars, 200 μm. Bar graphs indicate the means (±SE), Student’s t test [(E), (H), and (L)] or one-way ANOVA with Tukey post test [(C) and (K)]. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Article Snippet: For the therapeutic intervention, mice were placed on CCl 4 [0.5 ml/kg, intraperitoneally (ip), three times per week] for 4 weeks and treated with 250 μg of mouse anti–IL-17a (clone 17F3, BioXcell) antibody intraperitoneally in a volume of 200 μl (diluted in PBS) at the beginning of CCl 4 application and throughout the experiment or with the RORγt antagonist GSK805 (Sigma-Aldrich, cat. no. 5313690001) from weeks 2 to 4 daily at 10 mg/kg ip in corn oil.

Techniques: Imaging, Control, Cell Culture, Expressing

Journal: Frontiers in Aging Neuroscience

Article Title: Systems-level molecular and immunological evidence identifies Th17/Treg modulation as a key mechanism of CRSJ’s neuroprotection in Parkinson’s disease

doi: 10.3389/fnagi.2026.1764634

Figure Lengend Snippet: Expression of CX3CL1 and Th17 Cells in PD mice. (A) Co-localization of CX3CL1 and IL-17A in immunofluorescence images, along with quantitative analysis and serum CX3CL1 levels ( n = 4). (B) Western blot and quantitative analysis of CX3CL1 and IL-17A ( n = 5). # P < 0.05 vs. Model group; * P < 0.05 vs. Control group. CRSJ-L, low-dose Congrong Shujing Granules; CRSJ-M, medium-dose Congrong Shujing Granules; CRSJ-H, high-dose Congrong Shujing Granules.

Article Snippet: For Th17 staining, cells were labeled with FITC-anti-CD4 and APC-anti-IL-17A antibodies (Elabscience, No. E-AB-F1199E).

Techniques: Expressing, Immunofluorescence, Western Blot, Control